FRIDAY, February 28, 2003
Time: 1:30 - 2:30 PM
Constant Hall Room 1052

Title: Identifying Differentially Expressed Genes in a Microarray Experiment

Deepak Mav
Department of Mathematics & Statistics, Old Dominion University

In recent years microarrays have become a popular tool for studying expressions of thousands of genes simultaneously. Two kinds of microarray chips are in use, the spotted cDNA chip developed by Patrick Brown in the mid-1990s at Stanford University, and the oligonucleotide array first commercially released by Affimetrix Corp., in 1996. The primary focus of this talk will be the former, the spotted cDNA microarray. Here the experimental sample is labeled with red dye, and hybridized along with a reference sample labeled with green dye on a chip that contains in many cases more than tens and thousands of spots. Using a fluorescent microscope, the log (red-green) intensities are measured at each spot. We will discuss a statistical model for the analysis of these intensities to calculate the posterior odds of gene expressions. Some simulation results will also be presented for model validation.